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Table 1 Positive clones of transgenic hairy roots.

From: Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots

 

Number of root lines

 

Gene constructs

Total

Hygromycin-resistant

PCR-positive

Established root lines

AaPMT

25

17

9

P1 P2 P3 P5 P6 P7 P9 P12 P16

AaTRI

19

15

5

T3 T4 T5 T6 T12

AaPMT-AaTRI

46

36

21

PT2 PT3 PT4 PT5 PT6 PT7 PT10 PT12 PT13 PT15 PT17 PT18 PT19 PT20 PT21 PT22 PT24 PT25 PT26 PT27 PT28