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Figure 2 | BMC Biotechnology

Figure 2

From: Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots

Figure 2

DNA identification and gene transcripts analysis. A) PCR analyses of transgenic hairy root lines. Analyses for the presence of AaPMT (1269 bp) and AaTRI genes (1074 bp) in PT lines (1), AaPMT gene in P lines (2) and AaTRI gene in T lines (3), respectively. M, DL-2000 Marker. PC, positive control (vector contains corresponding gene). BC, blank control (hairy root generate from blank-vector transformation). B) Effects of transformation on the expression of related genes. AaPMT T and AaTRI T represent total gene of AaPMT and AaTRI respectively, AaPMT E and AaTRI E represent endogenous AaPMT and AaTRI respectively. C) Real-time fluorescence quantitative PCR analysis of the expressions of AaPMT and AaTRI in transgenic hairy roots.

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BMC Biotechnology

ISSN: 1472-6750

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