Table 1 Sampling points 4 and 5

Total Lipid content (mg/g DW)

33.41 ± 3.66

41.55 ± 4.64

27.13 ± 3.41

14:0

0.22 ± 0.02

0.43 ± 0.05

0.43 ± 0.04

16:0

26.53 ± 1.73

23.91 ± 1.49

22.56 ± 0.67

18:0

2.92 ± 0.30

4.10 ± 0.28

4.02 ± 0.27

18:1n-9

4.56 ± 0.92

5.53 ± 1.00

5.61 ± 0.72

18:1 n-7

1.53 ± 0.05

1.19 ± 0.09

1.03 ± 0.17

18:2n-6 (LA)

36.82 ± 0.99

41.39 ± 0.95

40.16 ± 1.55

18:3n-6 (GLA)

13.88 ± 0.78

10.48 ± 0.27

10.55 ± 0.53

18:3n-3 (ALA)

4.24 ± 0.20

3.54 ± 0.25

4.61 ± 1.75

18:4n-3 (SDA)

1.26 ± 0.45

0.68 ± 0.00

0.84 ± 0.30

20:0

0.29 ± 0.06

0.40 ± 0.08

0.37 ± 0.05

22:0

2.45 ± 0.31

2.58 ± 0.36

2.50 ± 0.32

24:0

1.94 ± 0.49

2.49 ± 0.28

2.72 ± 0.77

Unknown

2.36 ± 0.35

2.64 ± 0.08

2.65 ± 0.15

Fatty acids (% of total lipid content)

22.17 ± 4.23

23.96 ± 4.02

18.12 ± 2.43

GLA and SDA

15.14 ± 1.23

11.16 ± 0.27

11.39 ± 0.83

Saturated fatty acids

34.36 ± 1.67

33.90 ± 1.92

32.61 ± 0.99

Monoene fatty acids

7.07 ± 0.73

7.37 ± 0.82

7.17 ± 0.87

n-9

5.33 ± 0.66

6.04 ± 1.11

6.14 ± 0.79

n-6

50.70 ± 1.66

51.87 ± 0.97

50.72 ± 1.06

n-3

5.50 ± 0.53

4.22 ± 0.25

5.45 ± 2.05

n-3/n-6

0.11 ± 0.01

0.08 ± 0.00

0.11 ± 0.04

n-6 Δ6-Desaturation Index

0.27 ± 0.01

0.20 ± 0.01

0.21 ± 0.01

n-3 Δ6-Desaturation Index

0.23 ± 0.06

0.16 ± 0.01

0.15 ± 0.00

DBI

1.40 ± 0.06

1.35 ± 0.03

1.36 ± 0.04

Sampling point 5

HR E1.5

(25 days)

HR E1.16

(35 days)

HR E1.16 with Kn

(70 days)

Total Lipid content (mg/g DW)

35.00 ± 5.40

29.75 ± 1.63

34.34 ± 5.25

14:0

0.23 ± 0.02

0.27 ± 0.06

0.38 ± 0.08

16:0

25.04 ± 1.14

23.63 ± 0.45

25.23 ± 0.27

18:0

2.55 ± 0.21

4.06 ± 0.21

3.93 ± 0.18

18:1n-9

6.77 ± 1.10

6.12 ± 0.44

4.01 ± 0.24

18:1 n-7

1.55 ± 0.16

0.86 ± 0.23

0.99 ± 005

18:2n-6 (LA)

34.55 ± 1.97

42.89 ± 1.31

38.05 ± 0.55

18:3n-6 (GLA)

11.77 ± 1.63

10.05 ± 0.40

11.95 ± 0.31

18:3n-3 (ALA)

4.22 ± 0.34

3.69 ± 0.23

5.12 ± 0.26

18:4n-3 (SDA)

0.88 ± 0.21

0.58 ± 0.03

1.09 ± 0.22

20:0

0.27 ± 0.04

0.42 ± 0.02

0.46 ± 0.02

22:0

2.51 ± 0.35

2.59 ± 0.19

2.76 ± 0.09

24:0

2.08 ± 0.60

2.25 ± 0.45

2.69 ± 0.24

Unknown

6.08 ± 2.68

2.03 ± 0.44

2.87 ± 0.17

Fatty acids (% of total lipid content)

24.53 ± 4.16

26.86 ± 3.29

27.27 ± 9.10

GLA and SDA

12.65 ± 1.84

10.63 ± 0.43

13.04 ± 0.53

Saturated fatty acids

32.68 ± 2.21

33.22 ± 1.21

35.45 ± 0.07

Monoene fatty acids

9.54 ± 1,29

7.53 ± 0.55

5.48 ± 0.15

n-9

7.59 ± 1.15

6.66 ± 0.50

4.38 ± 0.08

n-6

46.32 ± 3.60

52.94 ± 1.67

50.00 ± 0.47

n-3

5.09 ± 0.53

4.28 ± 0.23

6.21 ± 0.41

n-3/n-6

0.11 ± 0.01

0.08 ± 0.00

0.12 ± 0.01

n-6 Δ6-Desaturation Index

0.25 ± 0.02

0.19 ± 0.00

0.24 ± 0.01

n-3 Δ6-Desaturation Index

0.17 ± 0.02

0.14 ± 0.01

0.17 ± 0.03

DBI

1.30 ± 0.08

1.37 ± 0.04

1.37 ± 0.00