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Table 4 Oligonucleotide primer sets and PCR amplicon properties

From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

No

Gene*

Chromosome

Amplicon

Primer (5'-3')

 

Name

No.

Position (Start-End)**

%GC

Fragment (bp)/name

Forward/reverse

Pr. 1

Thy-1

9

43854065-43854933

53.6

864/Fr. 1

ATGAACCCAGCCATCAGCG/GGGTAAGGACCTTGATATAGG

Pr. 2

NTAL

5

135085842-135086225

50.8

384/Fr. 2

CTAGGGAGCTGAGTGTTCTCA/GAACGGCTAGAACTACACAGAG

Pr. 3

NP_660313.1

16

613645-614375

71.5

731/Fr. 3

GGTCGCCGACATCCACTC/TGCTCCGGGAACAGAACCT

Pr. 4

Q8WZ58

11

2292033-2292813

71.7

781/Fr. 4

CCTGACCGTCCTGGCACA/CTGGCGAAATCTGCGAGTTC

Pr. 5

NP_060193.2

9

140174905-140175662

72.4

758/Fr. 5

CCCCTCACTCAGGTCGTGTTTT/CCCTCTGAGCCCCTTTCG

Pr. 6

Q8N4X1

16

2029023-2029774

72.6

752/Fr. 6

CGGTCCATCCCCTCATCG/ACCCCTCACGCCACCAC

Pr. 7

NP_001035158.1

16

33961629-33962363

73.1

735/Fr. 7

CGGCGAACCGGACATCC/GGCTCGTGAGGCGGGTCT

Pr. 8

Q8N1R6

13

111267817-111268622

73.3

806/Fr. 8

GACACGGCCCTGCTCC/GGGTGTGATTGAGCGAGTTG

Pr. 9

NM_020975

10

43572333-43572724

79.1

392/Fr. 9

CCCGCACTGAGCTCCTACAC/GGACGTCGCCTTCGCCATCG

  1. * Homo_sapiens LATESTGP database was used, except for Thy-1 and NTAL for which Mus_musculus LATESTGP database was used.
  2. ** Primer sets Pr. 3 - Pr. 9. are based on Ensembl release 56 - Sept 2009