1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

BMC Biotechnology

Table 3 Effect of the tested PCR mixes and selected DNA dyes on DNA polymerase fidelity

Sample	Colonies mutant/total	Template doubling^a	Mutation frequency^b	Error rate (× 10^-6)^c
Taq in mix I	362/5789	12.5	0.063	38.8
Taq in mix II	990/16342	10.9	0.061	43.0
Taq in mix III	2760/21433	12.4	0.129	80.0
Taq in mix IV	951/8273	11.7	0.115	75.6
KOD in mix K^d	30/25006	12.1	0.0012	0.76
Various DNA dyes in PCR mix II
SYTO-9	318/4845	9.1	0.066	46.0
SYTO-13	640/10087	9.2	0.063	44.7
EvaGreen	307/5607	9.2	0.054	38.6
LCGreen	211/4068	9.0	0.052	35.8
ResoLight	64/1243	9.1	0.052	36.2

^a Template doublings (d) were calculated using the equation 2^d = (amount of PCR product)/(amount of starting target).
^b Mutation frequency = (mutant colonies)/(total colonies).
^c Error rate = (mutation frequency)/(130 × d).
^d PCR mix provided by KOD enzyme manufacturer

ISSN: 1472-6750