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Table 3 Effect of the tested PCR mixes and selected DNA dyes on DNA polymerase fidelity

From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

Sample Colonies mutant/total Template doublinga Mutation frequencyb Error rate (× 10-6)c
Taq in mix I 362/5789 12.5 0.063 38.8
Taq in mix II 990/16342 10.9 0.061 43.0
Taq in mix III 2760/21433 12.4 0.129 80.0
Taq in mix IV 951/8273 11.7 0.115 75.6
KOD in mix Kd 30/25006 12.1 0.0012 0.76
Various DNA dyes in PCR mix II
SYTO-9 318/4845 9.1 0.066 46.0
SYTO-13 640/10087 9.2 0.063 44.7
EvaGreen 307/5607 9.2 0.054 38.6
LCGreen 211/4068 9.0 0.052 35.8
ResoLight 64/1243 9.1 0.052 36.2
  1. a Template doublings (d) were calculated using the equation 2d = (amount of PCR product)/(amount of starting target).
  2. b Mutation frequency = (mutant colonies)/(total colonies).
  3. c Error rate = (mutation frequency)/(130 × d).
  4. d PCR mix provided by KOD enzyme manufacturer