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Figure 3 | BMC Biotechnology

Figure 3

From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

Figure 3

Amplification of various GC-rich genomic DNA fragments from whole human blood. PCR mix II was supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (final concentrations; Mix II-PT) or 0.3 M trehalose, 0.24 M L-carnitine, and 0.4% Nonidet P-40 (Mix II-TCN). Various GC-rich templates from heparinized whole human blood (~100 copies of the template/μl of PCR mixture) were amplified in the two PCR mixes using primer sets specified in Table 4. Positions of the specific fragments (731-806 bp in size) are indicated on the right (Fr. 3-8). Lane L, DNA standard ladder with sizes indicated in kbp on the left. The numbers at the top indicate percentages of GCs in specific amplicons. A typical experiment of at least four performed is shown.

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