Figure 1
From: A suicide gene approach using the human pro-apoptotic protein tBid inhibits HIV-1 replication
Induction of cell death by tBid in HeLa SS6 cells and general architecture of the control vector pLRed(INS) 2 R and the suicide vector pLtBid(INS) 2 R. (A) Hela SS6 cells were transfected with pCMV-tBid and/or pEGFP-C1. 24 hours after transfection, cell death and GFP expression were determined by FACS analysis. Shown is a representative example of three independent experiments. Percentages of GFP-positive, PI-positive and GFP + PI-positive cells are given for each quadrant. (B) Schematic representation of pLRed(INS)2R and pLtBid(INS)2R. p17/p24 INS = INS-containing region from gag; RRE = Rev-responsive element. (C) FACS analysis of dsRed expression from pLRed(INS)2R without (left) and with Rev (right) in HeLa-Tat cells constitutively expressing Tat. A plasmid, driving GFP expression under the control of the CMV promoter, was transfected as a transfection/expression control (modified from reference [40]).