Table 2 Primers used for amplifying the mouse VH and VL genes.
Use | Primer | Sequence |
|---|---|---|
1st PCR upper | AP3dC-S | CGGTACCGCGGGCCCGGGATCCCCCCCCCCCCCDN |
2nd PCR upper | MCSAP3-S | CTTCGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGA |
IgG 1st PCR lower | mIgG1st-AS | ACCYTGCATTTGAACTCCTTGCC |
IgG 2nd PCR lower | mIgG2nd-AS | CTGGACAGGGATCCAGAGTTCCA |
IgK 1st PCR lower | mIgK1st-AS | ACTGCCATCAATCTTCCACTTGACA |
IgK 2nd PCR lower | mIgK2nd-AS | ACTGAGGCACCTCCAGATGTTAACT |