Figure 5From: Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cellsExpression of recombinant mouse antibodies. (A) The concentration of the recombinant antibodies in the supernatant of cultured 293FT cells was determined by sandwich ELISA. N indicates a negative control; all of the incubation steps were identical, except that non-transfected cell culture medium was used. (B) The specific activity of the recombinant antibodies was expressed as RLU/s/IgG (μg).Back to article page