Figure 1

Flow chart summarizing the production of recombinant antibodies from single plasma cells. 3' Homopolymer-tailed cDNA was synthesized from single plasma cells by droplet-based solid-phase cDNA synthesis. The VH and VL genes were amplified by two rounds of PCR. The unpurified VH and VL genes were directly inserted into the corresponding vectors by TS-HR. After the transformation, the bacteria were directly cultured in liquid medium, and pools of expression plasmids were extracted without screening single colonies for correct clones. The cognate pairs of the expression plasmids were co-transfected into 293FT cells. Two days after the transfection, the antibodies secreted into the cell culture medium were tested for reactivity by ELISA.