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Table 3 Comparison of AUCs derived with the MTRC-4 and MTRC-3 using different labeling protocols

From: An adaptable method using human mixed tissue ratiometric controls for benchmarking performance on gene expression microarrays in clinical laboratories

    

Variations in method

 

AUC

Dataset

Method

MTRC

designa

Kit

Total RNA

input

(μg)

Labeling

time

(hr)

Target

hybridized

(μg)b

 

4-fold

2-fold

1.5-fold

1A-4

1A

4

IVT Express

0.1

16

6.5

 

0.99

0.99

0.95

1B-4

1B

4

IVT Express

0.1-0.2

4

6.5

 

0.99

0.98

0.93

2A-4

2A

4

IVT

5

16

6.5

 

0.98

0.97

0.93

2A-3

2A

3

IVT

5

16

6.5

 

-

-

0.95

3A-4

3A

4

Ovation

0.02

1

2.2

 

0.99

0.97

0.87*

3B-4

3B

4

Ovation

0.02

1

0.55

 

0.99

0.98

0.89*

3C-3

3C

3

Ovation

0.1

1

0.55

 

-

-

0.88*

  1. aThe MTRC-4 design was tested with MTRC Batch 4. The MTRC-3 design was tested with MTRC Batch 5.
  2. bThe target is aRNA for Methods 1 and 2 and cDNA for Method 3.
  3. *Statistically different from italicized values (P < 0.01).
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BMC Biotechnology

ISSN: 1472-6750

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