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Figure 1 | BMC Biotechnology

Figure 1

From: Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivostudies

Figure 1

Expression of hIGF-I in transgenic rice. (A) Nucleotide sequence of the modified hIGF-I cDNA. The modified nucleotide is underlined and the change of codon was 28.6%. (B) The hIGF-I protein is predicted to contain 70 amino acids. (C - E) The recombinant hIGF-I expression constructs, driven by glutelin promoter, used for rice transformation. (C) The construct, pSB130/Gt1/hIGF-I (I) contained the modified hIGF-I alone. (D) The construct, pSB130/Gt1/SP/hIGF-I (SI) contained glutelin signal peptide only (E) whereas the pSB130/Gt1/SP/hIGF-I/KEDL (SIK) construct carried both the glutelin signal peptide and KDEL. (F) All the chimeric genes were ligated into the twin T-DNA binary vector, pSB130, for Agrobacterium transformation. The pSB130 vector, contains two T-DNAs, one flanking the gene of interest driven by Gt1 promoter while the other flanking the selectable marker, hygromycin phosphotransferase (HYG). (Abbreviations: RB - right border; LB - left border.)

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BMC Biotechnology

ISSN: 1472-6750

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