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Figure 4 | BMC Biotechnology

Figure 4

From: Developing a method for customized induction of flowering

Figure 4

Induction of FTa1 expression by ethanol vapour. Alc::FTa1 T3 transformants or control plants in LD conditions were either exposed (+ETOH), or not (-ETOH), to ethanol vapour. Transcript accumulation in the transgenic lines and control plants was measured using qRT-PCR with levels normalised to At2g32170. a) Relative transcript abundance of FTa1 after 10-day-old plants (TG1 to TG4 and controls) were exposed to ethanol vapour for 24 h (mean +/- SD of 3 PCR replicates is shown). Induction was started 4 h after dawn and plants were harvested 1 day later at the same time. b) Relative transcript abundance of endogenous Arabidopsis FT after ethanol induction as in (a) (mean +/- SD of 3 PCR replicates is shown). c) Time course of induction of FTa1 expression by ethanol vapour. Transcript accumulation was measured using qRT-PCR with levels normalised to At2g32170. Time course of accumulation of FTa1 after 16 day-old TG1 plants were exposed to ethanol vapour for 8, 12 or 24 h (mean +/- SD of qPCR on 2 biological replicates is shown). Induction was started at dawn.

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