Figure 1

Protein and mRNA sequence alignments and phylogenetic analysis of FT and TFL1 proteins. a) Alignment of selected full-length FT and TFL1 proteins from Medicago and Arabidopsis. Background black shading indicates identical amino acids shared in 50% or more sequences, gray indicates similar amino acids shared in 50% or more sequences. Black underlining marks key functional amino acid residues in FT (see text for details). b) Neighbour joining tree of selected FT and TFL1 full length proteins from 11 different plant species. The tree was rooted on AtBFT. Bootstrap values from 1000 replications are shown as a percentage above each branch. The Medicago proteins are highlighted with a gray box. c) Alignment of the sequence of the amiR-FT to Arabidopsis FT transcript (above) and to Medicago FTa1 mRNA (below). At Arabidopsis thaliana; Gm Glycine max, Hv Hordeum vulgare; Le Lycopersicon esculentum now Solanum lycopersicum; Lj Lotus japonicus, Mt Medicago truncatula Os Oryza sativa, rice, Pn Pharbitis nil, Ps Pisum sativum, Ta Triticum aestivum, Zm Zea mays. Gene identifiers (At) or Accession numbers are listed in the methods.