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Table 1 Yield, association rate and solubility of synthesized membrane proteins

From: Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system

  Proteins Anotation Number of TMD Molecular weight (kDa) Yield ( μg/150 μl reaction) Solubility (%) Association rates with liposomes (%) Yield × Association rates ( μg)
1 Itga1# Integrin 1 131 10.2 38.3 43.3 4.4
2 KCNJ8 Potassium inwardly-rectifying channel 2 48 4.6 22.6 72.6 3.3
3 KCNJ13 Potassium inwardly-rectifying channel 2 41 5.9 23.1 48.7 2.9
4 KCNJ15 Potassium inwardly-rectifying channel 2 43 12.5 10.5 58.8 7.3
5 P2RX1 Purinergic receptor 2 45 4.7 46.0 65.4 3.1
6 P2rx2# Purinergic receptor 2 55 5.0 40.4 58.3 2.9
7 P2RX4 Purinergic receptor 2 43 6.1 30.5 65.4 4.0
8 P2RX5 Purinergic receptor 2 47 5.5 50.0 58.2 3.2
9 GRIA2 Glutamate receptor 3 99 7.3 24.1 59.8 4.4
10 CACNG3 Voltage-dependent calcium channel 4 36 0.9 54.1 62.7 0.6
11 CACNG4 Voltage-dependent calcium channel 4 37 0.4 61.0 70.8 0.3
12 GABRA3 GABA receptor 4 55 11.0 17.1 58.1 6.4
13 GABRB1 GABA receptor 4 54 4.9 21.0 60.9 3.0
14 GABRD GABA receptor 4 51 8.4 12.5 58.8 4.9
15 GABRG1 GABA receptor 4 54 7.0 6.9 48.6 3.4
16 Glra1# Glycine receptor 4 52 5.4 17.1 67.6 3.6
17 AQP3 Aquaporin 6 32 0.4 21.4 66.6 0.3
18 EDNRA Endothelin receptor 7 49 11.1 8.3 56.6 6.3
19 EDNRB Endothelin receptor 7 50 3.9 28.3 68.3 2.7
20 GPR37 G protein-coupled receptor 37 7 67 0.5 33.0 58.8 0.3
21 HTR2B 5-Hydroxytryptamine receptor 7 54 2.2 57.0 55.8 1.2
22 P2RY2 Purinergic receptor 7 42 7.4 18.9 62.4 4.6
23 P2RY10 Purinergic receptor 7 39 7.5 14.0 61.2 4.6
24 P2RY11 Purinergic receptor 7 40 3.8 26.3 51.7 2.0
25 P2RY13 Purinergic receptor 7 38 8.3 12.7 64.8 5.4
26 P2RY14 Purinergic receptor 7 39 5.6 14.3 53.5 3.0
27 SLC1A7 Glutamate transporter 10 61 6.7 42.9 69.4 4.6
28 SLC6A3 Dopamine transporter 12 69 0.7 9.1 44.4 0.3
29 SLC6A13 GABA transporter, GABA 12 68 5.5 38.1 51.1 2.8
30 SLC6A18 Solute carrier family 6, member 18 12 71 13.1 15.3 48.1 6.3
31 Slc18a2# Vesicular monoamine transporter 12 56 10.9 27.1 56.3 6.2
32 SLC22A4 Organic cation transporter 12 62 4.4 33.1 60.2 2.6
33 SLC22A7 Organic anion transporter 12 60 0.8 56.2 62.7 0.5
34 SLC22A8 Organic anion transporter 12 60 5.6 36.0 56.9 3.2
35 SLC22A11 organic anion/cation transporter 12 60 7.1 14.1 62.7 4.5
36 SLC22A12 Organic anion/cation transporter 12 60 6.2 16.4 62.3 3.9
37 Slc5a1# Sodium/glucose cotransporter 14 73 10.6 21.8 53.4 5.7
38 SLC5A6 Sodium-dependent vitamin transporter 14 69 5.1 28.7 62.1 3.2
39 SLC5A10 Sodium/glucose cotransporter 14 62 4.1 42.2 47.5 1.9
40 Slc7a1# Cationic amino acid transporter 14 67 6.7 23.3 57.3 3.8
  1. # Mouse clone