Table 1 Yield, association rate and solubility of synthesized membrane proteins
|  | Proteins | Anotation | Number of TMD | Molecular weight (kDa) | Yield ( μg/150 μl reaction) | Solubility (%) | Association rates with liposomes (%) | Yield × Association rates ( μg) |
|---|---|---|---|---|---|---|---|---|
1 | Itga1# | Integrin | 1 | 131 | 10.2 | 38.3 | 43.3 | 4.4 |
2 | KCNJ8 | Potassium inwardly-rectifying channel | 2 | 48 | 4.6 | 22.6 | 72.6 | 3.3 |
3 | KCNJ13 | Potassium inwardly-rectifying channel | 2 | 41 | 5.9 | 23.1 | 48.7 | 2.9 |
4 | KCNJ15 | Potassium inwardly-rectifying channel | 2 | 43 | 12.5 | 10.5 | 58.8 | 7.3 |
5 | P2RX1 | Purinergic receptor | 2 | 45 | 4.7 | 46.0 | 65.4 | 3.1 |
6 | P2rx2# | Purinergic receptor | 2 | 55 | 5.0 | 40.4 | 58.3 | 2.9 |
7 | P2RX4 | Purinergic receptor | 2 | 43 | 6.1 | 30.5 | 65.4 | 4.0 |
8 | P2RX5 | Purinergic receptor | 2 | 47 | 5.5 | 50.0 | 58.2 | 3.2 |
9 | GRIA2 | Glutamate receptor | 3 | 99 | 7.3 | 24.1 | 59.8 | 4.4 |
10 | CACNG3 | Voltage-dependent calcium channel | 4 | 36 | 0.9 | 54.1 | 62.7 | 0.6 |
11 | CACNG4 | Voltage-dependent calcium channel | 4 | 37 | 0.4 | 61.0 | 70.8 | 0.3 |
12 | GABRA3 | GABA receptor | 4 | 55 | 11.0 | 17.1 | 58.1 | 6.4 |
13 | GABRB1 | GABA receptor | 4 | 54 | 4.9 | 21.0 | 60.9 | 3.0 |
14 | GABRD | GABA receptor | 4 | 51 | 8.4 | 12.5 | 58.8 | 4.9 |
15 | GABRG1 | GABA receptor | 4 | 54 | 7.0 | 6.9 | 48.6 | 3.4 |
16 | Glra1# | Glycine receptor | 4 | 52 | 5.4 | 17.1 | 67.6 | 3.6 |
17 | AQP3 | Aquaporin | 6 | 32 | 0.4 | 21.4 | 66.6 | 0.3 |
18 | EDNRA | Endothelin receptor | 7 | 49 | 11.1 | 8.3 | 56.6 | 6.3 |
19 | EDNRB | Endothelin receptor | 7 | 50 | 3.9 | 28.3 | 68.3 | 2.7 |
20 | GPR37 | G protein-coupled receptor 37 | 7 | 67 | 0.5 | 33.0 | 58.8 | 0.3 |
21 | HTR2B | 5-Hydroxytryptamine receptor | 7 | 54 | 2.2 | 57.0 | 55.8 | 1.2 |
22 | P2RY2 | Purinergic receptor | 7 | 42 | 7.4 | 18.9 | 62.4 | 4.6 |
23 | P2RY10 | Purinergic receptor | 7 | 39 | 7.5 | 14.0 | 61.2 | 4.6 |
24 | P2RY11 | Purinergic receptor | 7 | 40 | 3.8 | 26.3 | 51.7 | 2.0 |
25 | P2RY13 | Purinergic receptor | 7 | 38 | 8.3 | 12.7 | 64.8 | 5.4 |
26 | P2RY14 | Purinergic receptor | 7 | 39 | 5.6 | 14.3 | 53.5 | 3.0 |
27 | SLC1A7 | Glutamate transporter | 10 | 61 | 6.7 | 42.9 | 69.4 | 4.6 |
28 | SLC6A3 | Dopamine transporter | 12 | 69 | 0.7 | 9.1 | 44.4 | 0.3 |
29 | SLC6A13 | GABA transporter, GABA | 12 | 68 | 5.5 | 38.1 | 51.1 | 2.8 |
30 | SLC6A18 | Solute carrier family 6, member 18 | 12 | 71 | 13.1 | 15.3 | 48.1 | 6.3 |
31 | Slc18a2# | Vesicular monoamine transporter | 12 | 56 | 10.9 | 27.1 | 56.3 | 6.2 |
32 | SLC22A4 | Organic cation transporter | 12 | 62 | 4.4 | 33.1 | 60.2 | 2.6 |
33 | SLC22A7 | Organic anion transporter | 12 | 60 | 0.8 | 56.2 | 62.7 | 0.5 |
34 | SLC22A8 | Organic anion transporter | 12 | 60 | 5.6 | 36.0 | 56.9 | 3.2 |
35 | SLC22A11 | organic anion/cation transporter | 12 | 60 | 7.1 | 14.1 | 62.7 | 4.5 |
36 | SLC22A12 | Organic anion/cation transporter | 12 | 60 | 6.2 | 16.4 | 62.3 | 3.9 |
37 | Slc5a1# | Sodium/glucose cotransporter | 14 | 73 | 10.6 | 21.8 | 53.4 | 5.7 |
38 | SLC5A6 | Sodium-dependent vitamin transporter | 14 | 69 | 5.1 | 28.7 | 62.1 | 3.2 |
39 | SLC5A10 | Sodium/glucose cotransporter | 14 | 62 | 4.1 | 42.2 | 47.5 | 1.9 |
40 | Slc7a1# | Cationic amino acid transporter | 14 | 67 | 6.7 | 23.3 | 57.3 | 3.8 |