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Table 5 Ethylene glycol content (mol/l) of X. laevis oocytes expressing different amounts of cRNA encoding wild-type (WT) DrAqp3b, two DrAqp3b mutants, or HsAQP3, after immersion in hypertonic ethylene glycol solution

From: Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

  cRNA injected
  1 ng 20 ng
Water 0.11 ± 0.004 a
(6050 ± 220)
DrAqp3b-WT 0.24 ± 0.01 c
(14568 ± 607)
0.34 ± 0.02 d
(21284 ± 1250)
DrAqp3b-H53A/G54H/T85A 0.20 ± 0.01 b
(11980 ± 599)
0.29 ± 0.01 c
(17835 ± 615)
DrAqp3b-T85A 0.33 ± 0.02 d
(20328 ± 1232)
0.43 ± 0.01 e
(27305 ± 635)
HsAQP3 0.20 ± 0.01 b
(11900 ±595)
0.24 ± 0.01 b
(14448 ± 602)
  1. Data are the mean ± SEM (n = 12-18 oocytes) of two different experiments. Data in parenthesis are in pmol/oocyte/min. Values from the same column with different superscript are statistically significant (ANOVA, p < 0.05).