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Table 5 Ethylene glycol content (mol/l) of X. laevis oocytes expressing different amounts of cRNA encoding wild-type (WT) DrAqp3b, two DrAqp3b mutants, or HsAQP3, after immersion in hypertonic ethylene glycol solution

From: Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

 

cRNA injected

 

1 ng

20 ng

Water

0.11 ± 0.004 a

(6050 ± 220)

DrAqp3b-WT

0.24 ± 0.01 c

(14568 ± 607)

0.34 ± 0.02 d

(21284 ± 1250)

DrAqp3b-H53A/G54H/T85A

0.20 ± 0.01 b

(11980 ± 599)

0.29 ± 0.01 c

(17835 ± 615)

DrAqp3b-T85A

0.33 ± 0.02 d

(20328 ± 1232)

0.43 ± 0.01 e

(27305 ± 635)

HsAQP3

0.20 ± 0.01 b

(11900 ±595)

0.24 ± 0.01 b

(14448 ± 602)

  1. Data are the mean ± SEM (n = 12-18 oocytes) of two different experiments. Data in parenthesis are in pmol/oocyte/min. Values from the same column with different superscript are statistically significant (ANOVA, p < 0.05).
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BMC Biotechnology

ISSN: 1472-6750

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