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Table 4 Hydraulic conductivity (Lp) and ethylene glycol permeability (PEG) of X. laevis oocytes expressing wild-type (WT) DrAqp3b, two DrAqp3b mutants or HsAQP3, in hypertonic ethylene glycol solution

From: Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

  Lp (μm/min/atm) PEG (x 10-3 cm/min)
Water 0.36 ± 0.02 a 0.47 ± 0.03 a
DrAqp3b-WT (1 ng) 2.15 ± 0.14 c 17.59 ± 0.83 d
DrAqp3b-H53A/G54H/T85A (1 ng) 1.55 ± 0.13 b 13.06 ± 0.72 c
DrAqp3b-T85A (1 ng) 2.64 ± 0.15 d 25.49 ± 1.56 e
HsAQP3 (1 ng) 1.31 ± 0.08 b 10.73 ± 0.59 b
DrAqp3b-WT (20 ng) 2.63 ± 0.07 d 40.06 ± 1.22 c
DrAqp3b-H53A/G54H/T85A (20 ng) 2.27 ± 0.07 c 36.58 ± 1.74 c
DrAqp3b-T85A (20 ng) 3.24 ± 0.08 e 53.91 ± 2.55 d
HsAQP3 (20 ng) 1.96 ± 0.08 b 22.24 ± 0.95 b
  1. Values (mean ± SEM) for water- and aquaporin-injected (1 or 20 ng cRNA) oocytes are calculated from the data in Figure 6B and C. For each cRNA dose, data with different superscript are statistically significant (ANOVA, p < 0.05).