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Table 3 Hydraulic conductivity (Lp) of X. laevis oocytes expressing wild-type (WT) DrAqp3b, DrAqp3b mutants or HsAQP3 determined after 2 or 10 min in a sucrose solution

From: Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

 

Lp (μm/min/atm)

 

2 min

10 min

Water

1.40 ± 0.06 a

0.69 ± 0.03 a

DrAqp3b-WT

2.15 ± 0.06 c

0.89 ± 004 b

DrAqp3b-H53A/G54H/T85A

1.94 ± 0.08 b

0.88 ± 004 b

DrAqp3b-T85A

2.57 ± 0.07 d

1.02 ± 0.04 c

HsAQP3

1.93 ± 0.07 b

0.87 ± 0.03 b

  1. Values (mean ± SEM) for water- and aquaporin-injected (1 ng cRNA) oocytes are calculated from the data in Figure 6A. Data from the same column with different superscript are statistically significant (ANOVA, p < 0.05).
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BMC Biotechnology

ISSN: 1472-6750

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