Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsiciusing two different methods

Table 1 Purification summary of PCPEL2 expressed by pET system

Purification steps	Total protein (mg)	Yield (%)	Total activity^b (U)	Specific activity (U/mg protein)	Purification (fold)
Crude supernatant^a	702.5	100	15455	22	1
Ni-NTA affinity chromatography	20.2	2.88	3131	155	7.0
Superdex-200	6.0	0.85	4500	750	34.1

^a The starting material was 1 L of crude E. coli supernatant.
^b Enzyme activity was defined as the amount of enzyme that produced a change in absorbance of 0.001 at 232 nm/min, as determined by the formation of unsaturated uronide.

ISSN: 1472-6750