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Table 1 Purification summary of PCPEL2 expressed by pET system

From: Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsiciusing two different methods

Purification steps Total protein
Total activityb
Specific activity
(U/mg protein)
Crude supernatanta 702.5 100 15455 22 1
Ni-NTA affinity chromatography 20.2 2.88 3131 155 7.0
Superdex-200 6.0 0.85 4500 750 34.1
  1. a The starting material was 1 L of crude E. coli supernatant.
  2. b Enzyme activity was defined as the amount of enzyme that produced a change in absorbance of 0.001 at 232 nm/min, as determined by the formation of unsaturated uronide.