Figure 1

Solubilization of PCPEL2 expressed by pET system. Lane M, molecular mass marker; lanes 1 and 2, the supernatant of fusion protein after ultrasonication at pH 7.0 and 9.0 buffer (10 mM Tris-HCl, 150 mM NaCl); lane 3, the supernatant of fusion protein after ultrasonication with suspending buffer (10 mM Tris-HCl pH 10.3 containing 150 mM NaCl, 2 M urea, 10 mM imidazole and 5 mM β-mercaptoethanol). The over-expressed protein is indicated by the arrow.