Figure 3
From: Proximity ligation in situ assay for monitoring the global DNA methylation in cells
siRNA-mediated down-expression of Dnmt1 promoted the decrease of Dnmt1/PCNA interactions and the decrease of 5 mC in U251 cells. (A) Impact of the siRNA-Dnmt1 treatments on the expression of Dnmt1 (ELISA method according to our previous report [17]), on the 5 mC (ELISA method) and on the number of Dnmt1/PCNA interactions (P-LISA method). siRNA-Dnmt1 (sc-35204, Tebu-Bio, France) (B) Correlation between the 5 mC (5-methylcyctosine) number and the number of Dnmt1/PCNA interactions.