Figure 2
From: Proximity ligation in situ assay for monitoring the global DNA methylation in cells
Decrease of Dnmt1/PCNA interaction is associated with the decrease of the DNA methylation. (A) Dnmt1/PCNA P-LISA in non-tumor cells (MCF10A) and in tumor cells (MDA-MD-231 and Cal-51). Red dots symbolize Dnmt1/PCNA interactions. Nucleus/DNA are stained in blue via the use of DAPI. The number of Dnmt1/PCNA interactions is calculated from the analysis of 100 nuclei. (B) Evaluation of the degree of DNA methylation in cells by using 5 mC-ELISA. (C) Correlation between the 5 mC (5-methylcyctosine) number and the number of Dnmt1/PCNA interactions.