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Figure 1 | BMC Biotechnology

Figure 1

From: Proximity ligation in situ assay for monitoring the global DNA methylation in cells

Figure 1

Detection of endogenous Dnmt1/PCNA interactions using P-LISA. (A) Schematic representation of the Dnmt1/PCNA proximity ligation in situ assay (P-LISA). (B) Calibration of the microscopy (Axiovert 200 M Zeiss, Le Pecq, France) with ApoTome module. Left: picture of calibration performed with calibration balls (4 μm, Vue X-Y, Molecular Probes F36909). Right: picture of calibration performed with calibration balls (2.5 μm, Vue X-Z, Molecular Probes F36909). Bleu and red were merged in lateral and axial. (C) Decovolving of picture realized with calibration balls (140 nm). (D) Dnmt1/PCNA interactions were visualized in MCF10A cells. Red dots symbolize Dnmt1/PCNA interactions. Nucleus/DNA are stained in blue via the use of DAPI. Top left: ApoTome view, top right: orthogonal view, bottom: 3D views.

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