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Table 4 Primer sequences used for PCR amplification of coding regions of LOX and HPL genes for cloning into the pYES2 vector.

From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application

Genes Sequences Cloning sites
Nb-9-LOX Forward: 5'-CGGGGTACCAACACAATGTCTCTGGAGAAGATT-3' KpnI/NotI
  Reverse: 5'- ATTGCGGCCGCCTATATTGACACACTGTT-3'  
Cm-9/13-HPL Forward: 5'- CGCGGATCCTACACAATGTCTACTCCTTCTTCC-3' BamHI/XhoI
  Reverse: 5'- CCGCTCGAGTTAAACCATATCGGTTGC-3'  
Cl-13-HPL Forward: 5'- CGGGGTACCAACACAATGAAGGTCACCATGACC-3' KpnI/NotI
  Reverse: 5'- ATTGCGGCCGCTCAGTTGGTCCTTTGAAA-3'  
  1. The underlined nucleotide sequences indicate the sites of the restriction enzymes for insertion into multiple cloning sites of a plasmid.
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