From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application
Material
Amount
Linoleic acid
1.3 g (1.5 mM)
Yeast culture for extraction of Nb-9-LOX
260 ml* (234 units)
Citric acid
33 g (50 mM, pH 6.0)
Cysteine
3.3 g (6 mM)
Yield
72%