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Table 3 Starting material needed for production of 1 g of 9-HOD.

From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application

Material Amount
Linoleic acid 1.3 g (1.5 mM)
Yeast culture for extraction of Nb-9-LOX 260 ml* (234 units)
Citric acid 33 g (50 mM, pH 6.0)
Cysteine 3.3 g (6 mM)
Yield 72%
  1. * The number was calculated from the data shown in Table 1.