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Table 2 Formation of C6- and C9-aldehydes from linoleic acid in different combinations of LOX and HPL enzymes.

From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application

  n-Hexanal
(nmol mg-1protein*)
(3Z)-Nonenal
(nmol mg-1protein*)
(2Z)-Nonenal
(nmol mg-1protein*)
(2E)-Nonenal
(nmol mg-1protein*)
Nb-Cl 48 ± 28 1.9 ± 1.6 0.41 ± 0.37 0.17 ± 0.15
Nb-Cm 30 ± 13 2527 ± 316 360 ± 100 430 ± 143
  1. * Crude extracted protein.
  2. Nb, yeast expressed Nb-9-LOX; Cl, yeast expressed Cl-13-HPL; Cm, yeast expressed Cm-9/13-HPL. The amount of n-hexanal and various nonenal isomers were determined using standard curves calculated from various known concentrations of n-hexanal and (2E)-nonenal against the mass peak areas which were recorded by SPME-GC-MS, respectively. Each value is the average and standard error of three replicates.
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