Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamiana for biotechnological application

Table 2 Formation of C₆- and C₉-aldehydes from linoleic acid in different combinations of LOX and HPL enzymes.

	n-Hexanal (nmol mg^-1protein*)	(3Z)-Nonenal (nmol mg^-1protein*)	(2Z)-Nonenal (nmol mg^-1protein*)	(2E)-Nonenal (nmol mg^-1protein*)
Nb-Cl	48 ± 28	1.9 ± 1.6	0.41 ± 0.37	0.17 ± 0.15
Nb-Cm	30 ± 13	2527 ± 316	360 ± 100	430 ± 143

* Crude extracted protein.
Nb, yeast expressed Nb-9-LOX; Cl, yeast expressed Cl-13-HPL; Cm, yeast expressed Cm-9/13-HPL. The amount of n-hexanal and various nonenal isomers were determined using standard curves calculated from various known concentrations of n-hexanal and (2E)-nonenal against the mass peak areas which were recorded by SPME-GC-MS, respectively. Each value is the average and standard error of three replicates.

ISSN: 1472-6750