Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application

Table 1 Effect of substrate concentration on the yield of 9-HPOD from linoleic acid catalyzed by Nb-9-LOX-yeast protein extracts prepared from 1 ml of cell culture in 20 min.

The amount of 9-HPOD was determined using a standard curve calculated from various known concentrations of 9-HPOD against the UV peak areas which were recorded at 234 nm by LC-MS. Each value is the average and standard error of two replicates.