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Table 1 Effect of substrate concentration on the yield of 9-HPOD from linoleic acid catalyzed by Nb-9-LOX-yeast protein extracts prepared from 1 ml of cell culture in 20 min.

From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application

Linoleic acid (μmol) 9-HPOD (μmol) Yield (%)
12 11.8 ± 0.4 98
15 14.4 ± 0.6 96
18 17.9 ± 1.5 99
24 20.2 ± 3.0 84
30 19.7 ± 0.7 66
  1. The amount of 9-HPOD was determined using a standard curve calculated from various known concentrations of 9-HPOD against the UV peak areas which were recorded at 234 nm by LC-MS. Each value is the average and standard error of two replicates.
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