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Figure 3 | BMC Biotechnology

Figure 3

From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological application

Figure 3

Western blot analysis. Total proteins (20 μg) were isolated from yeast cells expressing Nb-9-LOX which were harvested at time points corresponding to 0, 4, 8, and 24 hours after galactose induction and separated on a 12% SDS/PAGE gel (A), then transferred to a PVDF membrane and LOX detected with a soybean-LOX antibody (B). The expressed Nb-9-LOX was indicated by arrows (→). M: Marker.

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