Figure 3From: Cloning and characterization of a 9-lipoxygenase gene induced by pathogen attack from Nicotiana benthamianafor biotechnological applicationWestern blot analysis. Total proteins (20 μg) were isolated from yeast cells expressing Nb-9-LOX which were harvested at time points corresponding to 0, 4, 8, and 24 hours after galactose induction and separated on a 12% SDS/PAGE gel (A), then transferred to a PVDF membrane and LOX detected with a soybean-LOX antibody (B). The expressed Nb-9-LOX was indicated by arrows (→). M: Marker.Back to article page