The effect of wounding on Nb-9-LOX gene expression. Leaves of N. benthamiana were infiltrated with buffer and were harvested at 30 min, 1 h, 2 h, 6 h, 24 h, and 48 h after wounding treatment. Quantitative real-time RT-PCR analysis was performed using Nb-9-LOX and 18S-26S interspacer gene specific primers, the latter used as internal control for normalization. Values are means ± SEM of three different evaluations carried out with two sets of cDNAs. Column C, untreated N. benthamiana; column V, tobacco leaf treated with TMV vectors.