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Figure 2 | BMC Biotechnology

Figure 2

From: A receptor fusion protein for the inhibition of murine oncostatin M

Figure 2

Inhibitory activities and specificity of mOSM-RFPs. (A) MEF were stimulated for 20 min with mOSM (5 ng/ml), mLIF (5 ng/ml) or mIL-6 (20 ng/ml) and murine soluble IL-6 receptor (sR, 500 ng/ml) in the presence of 5% of 10-fold concentrated supernatants of HEK293 cells expressing the respective mOSM-RFPs (resulting in a 6-fold molar surplus of mOSM-RFPs over mOSM) or of mock-transfected cells as indicated. (B) MEF were stimulated for 20 min with varying amounts of mOSM in the presence of 5% of 10-fold concentrated supernatants of HEK293 cells expressing mOSM-RFP or mOSM-RFP+D1 or of mock-transfected cells as indicated. Cellular lysates were analyzed for tyrosine phosphorylation of STAT3 and total STAT3 by Western blotting. (C) Fao hepatoma cells were stimulated for the indicated times with mOSM (5 ng/ml) in the presence of 2% of 10-fold concentrated supernatants of HEK293 cells expressing mOSM-RFP or corresponding supernatant of mock-transfected cells as indicated. Cellular lysates were analyzed for tyrosine phosphorylation of STAT3 and total STAT3 by Western blotting.

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