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Table 2 Short term vapor storage of vitrified embryos on different carriers to simulate transport conditions

From: Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport

  Cleavage Stage Vitrification
Carrier Cryoloop
(n = 40)
HSV Straw
(n = 35)
Cryotip
(n = 40)
Storage Condition LN2 Vapor LN2 Vapor LN2 Vapor
Survival (%) 100 100 100 100 100 100
Development to blastocyst after 48 hours (%) 100 100 100 93 100 100
Total blastomeres a (mean ± SD) 82.21 ± 13.28 89.18 ± 18.52 87.20 ± 10.67 88.5 ± 9.55 81.10 ± 14.09 75.53 ± 17.62
  Blastocyst Stage Vitrification
Carrier Cryoloop
(n = 41)
HSV straw
(n = 40)
Cryotip
(n = 35)
Storage Condition LN2 Vapor LN2 Vapor LN2 Vapor
Survival (%) 100 100 100 100 100 100
Re-expansion (%) 90 81 95 85 80 85
Total blastomeres a (mean ± SD) 108 ± 18 96 ± 19 96 ± 20 90 ± 20 96 ± 4 86 ± 19
  1. a Total cell count at termination of experiment for both Vit-CL and Vit-BL on day 5
  2. No significant difference in survival, development, re-expansion or cell number after short term vapor storage as compared to liquid nitrogen
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