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Table 1 Vitrification of cleavage and blastocyst stage embryos using open and closed carriers

From: Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport

Cleavage Stage Vitrification

Carrier

Total embryos

(n)

Recovery

(%)

Survival

(%)

Blastocyst formation after

48 hour culture (%)

Total blastomeres

(mean ± SD)

% DNA Damage

(mean ± SD)

Cryoloop

60

100

100

95

81.9 ± 14.0

1.85 ± 2.05

HSV

52

100

100

94

82.5 ± 15.6

2.06 ± 1.50

Cryotip

67

85*

100

98

78.6 ± 17.9

2.12 ± 2.04

Blastocyst Stage Vitrification

Carrier

Total embryos

(n)

Recovery

(%)

Survival

(%)

Re-expansion

(%)

Total blastomeres

(mean ± SD)

% DNA Damage **

(mean ± SD)

Cryoloop

44

100

100

100

86.4 ± 25.8

4.36 ± 2.72

HSV

55

100

100

100

85.9 ± 23.7

3.34 ± 2.79

Cryotip

52

75 *

79

79

88.0 ± 19.2

3.41 ± 2.66

  1. * Significantly lower recovery than with other carriers. P = 0.0001
  2. ** Percent DNA damage was higher in embryos vitrified at the blastocyst versus
  3. cleavage stage (P < 0.0001), regardless of the type of carrier