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Table 1 Vitrification of cleavage and blastocyst stage embryos using open and closed carriers

From: Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport

Cleavage Stage Vitrification
Carrier Total embryos
(n)
Recovery
(%)
Survival
(%)
Blastocyst formation after
48 hour culture (%)
Total blastomeres
(mean ± SD)
% DNA Damage
(mean ± SD)
Cryoloop 60 100 100 95 81.9 ± 14.0 1.85 ± 2.05
HSV 52 100 100 94 82.5 ± 15.6 2.06 ± 1.50
Cryotip 67 85* 100 98 78.6 ± 17.9 2.12 ± 2.04
Blastocyst Stage Vitrification
Carrier Total embryos
(n)
Recovery
(%)
Survival
(%)
Re-expansion
(%)
Total blastomeres
(mean ± SD)
% DNA Damage **
(mean ± SD)
Cryoloop 44 100 100 100 86.4 ± 25.8 4.36 ± 2.72
HSV 55 100 100 100 85.9 ± 23.7 3.34 ± 2.79
Cryotip 52 75 * 79 79 88.0 ± 19.2 3.41 ± 2.66
  1. * Significantly lower recovery than with other carriers. P = 0.0001
  2. ** Percent DNA damage was higher in embryos vitrified at the blastocyst versus
  3. cleavage stage (P < 0.0001), regardless of the type of carrier
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