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Figure 4 | BMC Biotechnology

Figure 4

From: Vitrification in Open and Closed Carriers at Different Cell Stages: Assessment of Embryo Survival, Development, DNA Integrity and Stability during Vapor Phase Storage for Transport

Figure 4

Fluorescent micrographs with representative images of DNA damage detected in vitrified-warmed mouse blastocysts. Nuclei of blastomeres stained with DAPI showing blue fluorescence. Nuclei with DNA strand breaks were labeled using the TUNEL assay. Damaged cells bound TMR-red dUTP and exhibited red fluorescence. A-C: Blastocysts vitrified on CryoTip and stored in LN2. TUNEL staining was performed 3 hours after warming. D-E: Blastocysts vitrified on CryoTip and stored in vapor phase under transport conditions. Stained 3 hrs post warming. G-Blastocyst vitrified on cryoloop and stored in LN2 (3 hrs post warming). H-Cleavage stage embryo vitrified on cryoloop and stored in LN2. TUNEL labeling 48 hours after warming.

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