Table 2 The piggyBac and Tol2 hotspots in the HEK 293 genome
Transposon | Target ID | Targeted sequence | Times | Position | Gene context | Targeted Gene | Near gene (distance bp) | Far gene (distance bp) |
|---|---|---|---|---|---|---|---|---|
piggyBac | B87-5/B89-3 | TTAAATAAAGATAATAATACTAACCATGGCA | 2 | 3p14.3 | INTRONIC | FLNB | Â | Â |
| Â | B89-4/B77-4 | TTAAAGACCCTGTCTCTTAAAAAAAAAAAAA | 2 | 16p11.2 | 3'UTR | MLAS | Â | Â |
| Â | B38-4/B102-2 | TTAAATAAAAAAGAACAGATATTTGAAATTG | 2 | 14q23.3 | INTRONIC | GPHN | Â | Â |
| Â | B71-1/B109-3 | TTAAATTCCAGGTTTCTCAAAGAAAGCTTGT | 2 | 20p12.3 | INTERGENIC | Â | BC043288(219296) | BMP2 (347908) |
| Â | B75-4/B92-1 | TTAAAGAAACAAGTTAACACCGAAGCCAGAG | 2 | 17q24.1 | INTERGENIC | Â | FLJ32065 (2686) | LRRC37A3 (101620) |
Tol2 | T47-2/T48-3 | TGTTCCGCTCCTGGTGCGGGCCGAGACCCGG | 2 | 1q21.2 | INTRONIC | ZNF687 | Â | Â |
| Â | 111-2/T119-1 | TATGTGTAATAATGGAGGTATGTACAACAT | 2 | 3p24.3 | INTERGENIC | Â | SGOL1 (385347) | HPX-42 (834010) |
| Â | T14-3/T17-1 | AGAATAGGTATTTCTTTTTTTCTTCTTATC | 2 | 5q33.2 | INTERGENIC | Â | C5orf3 (87465) | GRIA1 (92385) |
| Â | T2-1/T3-2 | TCCACCACAGCATGAGTTAAACCAAAGTCT | 2 | 7q11.23 | INTERGENIC | Â | POMZP3 (2680) | hPMSR6 (350693) |
| Â | T1-3/T4-1 | CCTGCCCAGCTCGTAAAAGGATGCTCACCT | 2 | 8q22.3 | INTERGENIC | Â | GRHL2 (694) | NACAP1 (122197) |
| Â | TB7-3/T113-1/T115-4/T120-2 | AATTTATCCATTTCTTCTAGATTTTCTAGT | 2 | 20p13 | INTRONIC | SIRPD | Â | Â |