The activity of genes which are close to or located at the site repeatedly targeted by Tol2 or piggyBac. The upper panel is a histogram showing the ratio of gene expression level between the housekeeping gene, GAPDH and the gene of interest that is either repeatedly targeted by piggyBac or Tol2, or is located within a 10-kb interval of piggyBac or Tol2 hotspots as measured by Q-RT-PCR. A set of neural genes (MK-1, NRGN, and SYGR4) with a high level to no expression in HEK 293 cells is also served as references. The lower panel is a DNA-agarose gel image of a representative Q-RT-PCR reaction showing the PCR products at the end of the 30th cycle. Genes targeted repeatedly by piggyBac: FLNB; GPHN; and MLAS. Genes near the piggyBac hotspot: FLJ32065. Genes targeted repeatedly by Tol2: SIRPD and ZNF687. Genes near the Tol2 hotspot: POMZP and GRHL2.