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Table 3 List of test proteins used to validate the groEL1ΔC expression strain

From: Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔCexpression strain

ORF

 

Annotation

 

Description

Expressed ...

 

Mol. Mass (kDa)

Rv2109c

Rv2110c

 

PrcA

PrcB

 

α- and β-subunit of the mycobacterial proteasome (α7β7β7α7 subunit organisation)

Using native operon content, producing a 730 kDa multimeric complex

 

26.8

30.3

Rv3285

Rv3280

 

AccA3

AccD5

 

α- and β-subunit from acyl-CoA carboxylase AccD5-AccA3 complex (α3β3β3α3 subunit organisation)

As monomeric proteins, mixed to form a acyl-CoA carboxylase complex of 740 kDa

 

63.8

59.4

Rv3874

Rv3875

 

CFP10

ESAT6

 

Potential virulence factor CFP10-ESAT6 complex

Using native operon content, producing a heterodimeric (1:1) complex

 

10.8

9.9

Rv2523c

 

ACPS

 

Holo-acyl-carrier protein synthase

As monomeric protein

 

14