Table 2 Primers used in this study
From: Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔCexpression strain
Primer | Sequence (5'-3') | Location 5' | Relative to |
|---|---|---|---|
Msmeg1583-F1.2 | GCGCCTTAAGCGACTGGGATCGCGAGAAGCTGC | +1067 | Msmeg1583 |
Msmeg1583-R1 | GCGCTCTAGACTCGTCCTCGTCGGCCGGCTTG | +1587 | Msmeg1583 |
Msmeg1583-F2 | GCGCAAGCTTGATCCATTTCACGCGACACCCCC | +1620 | Msmeg1583 |
Msmeg1583-R2.1 | GCGCACTAGTGGTGTTCGATCGTCTGGCCGATG | +2176 | Msmeg1583 |
accD5E5-F | GATCTCATGAGTATGACAAGCGTTACC G | +1 | Rv3280 |
accD5E5-R | GTCAAAGCTTTTATCGGCGCATGTGCG | +2161 | Rv3280 |
accA3-F | GATCCCATGGGTATGGCTAGTCACGCC | +2 | Rv3285 |
accA3-R | GTCAAAGCTTTTACTTGATCTCGGCGAGC | +1803 | Rv3285 |
Rv2523-F | CATGCCATGGGCATCGTCGGTGTGGGG | +1 | Rv2523 |
Rv2523-R | CCCAAGCTTACGGGGCCTCCAGGATGGC | +391 | Rv2523 |