Table 1 Plasmids and constructs used in this study

pJV53

Che9c recombination proteins under control of the acetamidase promoter in pLAM12

[9]

pYUB854

Hyg^R cassette flanked by γδ-res sites and 2 MCSs

[10]

pGH542

Expressing an γδ resolvase and tetracycline resistant

[11]

pEN15

pYUB854 with a 520 bp fragment harbouring groEL1 (+1067/+1587, relative to groEL1) inserted upstream of the Hyg^R cassette and a 555 bp fragment downstream of groEL1 including the STOP codon of groEL1, inserted downstream of the Hyg^R cassette

This paper

pMyNT

Mycobacterial overexpression vector

Geerlof et al., unpublished data

pMyNT/PrcA-B

Rv2109-2110 in pMYNT, Rv2110 is N-terminally his-tagged

[12]

pMyNT/AccD5E5

Rv3280-3281 in pMYNT. Only his-tagged Rv3280 seems to express using this construct.

This paper

pMyNT/AccA3

Rv3285 in pMyNT

This paper

pMyNT/CFP10-ESAT6

Rv3874-3875 in pMYNT, Rv3874 is N-terminally his-tagged

[12]

pMyNT/ACPS

Rv2523 in pMYNT

This paper