Figure 5From: Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔCexpression strainKinetics of AccD5-AccA3 isolated from M. smegmatis groEL1ΔC. Carboxylation activity of the acyl-CoA carboxylase AccD5-AccA3 complex isolated from groEL1ΔC was measured using an enzyme-coupled reaction with propionyl-CoA as substrate, providing Km = 0.1469 mM and Vmax = 28.5 mOD/min.Back to article page