Figure 4
From: Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔCexpression strain
Proteins isolated from both strains give virtually identical CD spectra. CD spectra of the multimeric proteasome complex PrcA-B (A), the dodecameric acyl-CoA carboxylase AccD5-AccA3 complex (B), CFP10-ESAT6 heterodimer (C), and monomeric protein ACPS (D) expressed in M. smegmatis mc2155 (WT = black) and M. smegmatis groEL1ΔC (grey) are virtually identical. For A and B, a concentration between 170 and 200 nM was used while for C and D, concentrations were between 5 and 10 μM.