Figure 3
From: Improved mycobacterial protein production using a Mycobacterium smegmatis groEL1ΔCexpression strain
GroEL1ΔC is absent from protein samples purified from M. smegmatis groEL1ΔC. SDS-PAGE analysis of protein samples isolated from cells expressing either CFP10-ESAT6 heterodimer (1), a holo-acyl-carrier protein synthase (ACPS) (2), the β- and α-subunit D5 (3) and A3 (4) of the acyl-CoA carboxylase AccD5-AccA3 complex, the PrcA-B complex (5) or the empty expression vector (empty pMyNT) (6). a, proteins expressed in M. smegmatis mc2155; b, proteins expressed in M. smegmatis groEL1ΔC. Purified proteins of interest are labelled with #. Visible presence of GroEL1 is depicted with *. Squares identify the bands excised for peptide mass fingerprinting. GroEL1 = 56 kDa.