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Figure 6 | BMC Biotechnology

Figure 6

From: Rapid obtention of stable, bioluminescent tumor cell lines using a tCD2-luciferase chimeric construct

Figure 6

Western blot analysis of recombinant luciferase and rat CD2 proteins in stable lymphoid and epithelial transfectants. Protein extracts were prepared from stably transfected Daudi and Hela cells as well as from the corresponding control cells. After gel separation and membrane transfer, these extracts were analysed using antibodies directed to the luciferase protein or the rat CD2.The tCD2-luc2 chimera, the wild-type luciferase 2 and the truncated rat CD2 are pointed out by a star, an arrowhead and an arch, respectively. The western blot membranes were restained with an anti-actin for loading control. A minor cleaved fragment of the tCD2-luc2 protein with a size identical to the wild-type luciferase 2 is detected at a low level in the extract of Hela cells transfected with the tCD2-luc2 fusion gene.

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