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Figure 5 | BMC Biotechnology

Figure 5

From: Rapid obtention of stable, bioluminescent tumor cell lines using a tCD2-luciferase chimeric construct

Figure 5

Luciferase activity in stable lymphoid and epithelial transfectants carrying recombinant luciferase constructs. Luciferase activity was assessed in protein extracts from Daudi and HeLa cells with stable expression of monocistronic (tCD2-luc2) or bicistronic (luc2-IRES-tCD2) luciferase constructs. The substrate was D-luciferin (Promega luciferase assay system, Promega). Light measurements were done on a Microlumat LB96P luminometer (Berthold, Thoiry, France). This experiment was repeated thrice; error bars represent SD of three experiments (n = 3). Error bars are not apparent above rectangles corresponding to Daudi luc2-IRES-tCD2 and Hela tCD2-luc2 because experimental data for these conditions were very consistent (very small error bars are not apparent for this size of the graph using the Microsoft Excel software).

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