Figure 1
Luciferase activity in 293 cells following short-term transfection of the wild-type luciferase 2 and tCD2-luciferase 2 genes. Recipient epithelial cells (293) were seeded at 2.105/well in 6-well plates and transfected using the Turbofect reagent (Fermentas) with increasing concentrations of plasmid DNA encoding either for the wild-type luc2 or the tCD2-luc2 chimera. They were grown in situ for 48 h prior to protein extraction and luciferase assay. For each condition, luciferase activity was normalized according to cell counts made on a fraction of the cells collected for the luciferase assay. This experiment was repeated six times (n = 6). Error bars represent SD of 6 experiments. Luciferase activities are significantly different for the two genes when using 500 ng of transfected plasmid (Mann&Whitney test, p < 0.05).