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Figure 5 | BMC Biotechnology

Figure 5

From: Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics

Figure 5

Comparison of oxygen transfer rate (OTR) and online pH of E. coli BL21 pRSET eYFP-IL6 during growth in Wilms-MOPS medium with different buffer concentrations and controlled-release discs. With 0.2 M MOPS buffer OTR () and pH (black line), with 0.1 M MOPS buffer and 3 controlled-release discs, OTR (▲) and pH (grey line). Experimental conditions: Wilms-MOPS medium with 20 g/L glucose, 37°C; 10 mL filling volume; shaking diameter (d0) 50 mm; 350 rpm; OD600,α = 0.5; pH0 = 7.5; controlled-release discs: D = 15 mm; H = 1.1 mm; 30% (w/w) Na2CO3.

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