Figure 3

Oxygen transfer rate (OTR) of E. coli BL21 pRSET eYFP-IL6 during growth in Wilms-MOPS medium with different buffer concentrations. With 0.2 M MOPS buffer (◇), with 0.1 M MOPS buffer (Δ), with 0.05 M MOPS buffer (●), without MOPS buffer (■); experimental conditions: Wilms-MOPS medium with 20 g/L glucose, 37°C; 10 mL filling volume; shaking diameter (d₀) 50 mm; 350 rpm; OD_600,α = 0.5; pH₀ = 7.5.