Figure 3

Functionality and efficacy of lentiviral destination vectors. A) H6c7 cells were transduced with viruses generated using dsRed2 destination expression vectors carrying EGFP or hygro selection markers. Following selection/sorting cells were infected with Adeno-Cre and subjected to flow cytometry 72 hours post infection. Red fluorescent profiles are compared between infected and non-infected cells. B) Virus titers of lentiviruses expressing increasing insert size as measured on 293T cells C) hTERT is overexpressed and D) active in H6c7 cell lines stably transduced with plko.YFP.hTERT.ON lentivirus.