Figure 1

Schematic diagram of shRNA/cDNA lentiviral expression platform. This system consists of two entry vectors, pENTR.hU6.hH1 and pENTR.CMV.ON and six lentiviral destination vectors containing different selection markers. Entry vector insert is flanked by attL sites used for recombination with attR sites found in the lentiviral destination vectors. C/LTR, fusion between the CMV IE gene enhancer and the promoter of the HIV 5'/LTR; ψ, RNA packaging signal; hPGK, human phosphoglycerate kinase eukaryotic promoter; CMV, cytomegalovirus promoter; SV40, simian-virus 40 promoter, puro, puromycin resistance gene; hygro, hygromycin resistance gene;; YFP, yellow fluorescent protein; EGFP, enhanced green fluorescent protein; dsRed2, red fluorescent protein; SIN/LTR, 3' self inactivating long terminal repeat; RRE, Rev response element; WPRE, 600-bp post-transcriptional regulatory element from Woodchuck hepatitis virus.