Figure 5
Influence of induction time on formation of EcFbFP under (A) unlimited (3000 mg/L K 2 HPO 4 ) and (B) limited (150 mg/L K 2 HPO 4 ) conditions. The measurements were performed with the Robo-Lector with a signal gain factor of 70. Each curve represents the EcFbFP production from duplicate cultures (n = 2) for every different time of induction. The relative standard deviation of each curve did not exceed ± 10%. The cultivation conditions were as follows: 37°C, shaking diameter 3 mm, shaking frequency 950 rpm, filling volume 200 μL, modified Wilms & Reuss medium (7.5 g/L Glucose), induction with IPTG (final concentration 0.1 mM) at 1 to 9 h.