Figure 4From: Utilizing high-throughput experimentation to enhance specific productivity of an E.coliT7 expression system by phosphate limitationInfluence of phosphate limitation on growth (A) and recombinant protein production (B). The measurements (scattered light as an indicator for biomass, panel (A); EcFbFP fluorescence as an indicator for total volumetric yield, panel (B)) were conducted in the BioLector prototype with a signal gain factor of 25. Each of the four curves shown represent the mean values (n = 12) of cultures grown in media (modified Wilms & Reuss media with 7.5 g/L glucose) containing phosphate at two different concentrations: 3000 mg/L K2HPO4 (unlimited conditions, solid blue lines) and 150 mg/L K2HPO4 (phosphate-limited conditions, dotted orange lines). The relative standard deviation of each curve did not exceed ± 10%. The cultivation conditions were as follows: 37°C, shaking diameter 3 mm, shaking frequency 950 rpm, filling volume 200 μL, induction with IPTG (final concentration 0.1 mM) after 9.5 h (indicated by arrows).Back to article page