Influence of phosphate limitation on growth (A) and recombinant protein production (B). The measurements (scattered light as an indicator for biomass, panel (A); EcFbFP fluorescence as an indicator for total volumetric yield, panel (B)) were conducted in the BioLector prototype with a signal gain factor of 25. Each of the four curves shown represent the mean values (n = 12) of cultures grown in media (modified Wilms & Reuss media with 7.5 g/L glucose) containing phosphate at two different concentrations: 3000 mg/L K2HPO4 (unlimited conditions, solid blue lines) and 150 mg/L K2HPO4 (phosphate-limited conditions, dotted orange lines). The relative standard deviation of each curve did not exceed ± 10%. The cultivation conditions were as follows: 37°C, shaking diameter 3 mm, shaking frequency 950 rpm, filling volume 200 μL, induction with IPTG (final concentration 0.1 mM) after 9.5 h (indicated by arrows).